NAD+ 500mg

What this is

Nicotinamide adenine dinucleotide — a coenzyme central to cellular energy metabolism, present in every living cell. Worth saying plainly up front: NAD+ is a dinucleotide, not a peptide. It sits in our catalog next to a lot of peptides, but chemically it is a different kind of molecule entirely, and it behaves differently in solution and in storage.

NAD+ is not exotic. It is one of the most fundamental molecules in biochemistry — it has a CAS registry number (53-84-9) that predates most of the peptide catalog by decades, and it shows up in undergraduate textbooks as the electron carrier in cellular respiration. What makes it interesting to current research is not that it is new, but that the question of how its cellular pool changes over time has become one of the busiest areas in cellular-aging work.

This is the 500mg vial. There is also a 1000mg vial for larger-volume work.

Research profile

Studied extensively in cellular-aging and metabolism research as a model for mitochondrial energy production, sirtuin activity, and NAD-dependent repair pathways.

Three research threads account for most of the published NAD+ work, and it is useful to know which one you are reading:

Mitochondrial energy production. NAD+ and its reduced form NADH shuttle electrons through the respiratory chain. Research here tends to be about redox ratio — the balance between the two forms — rather than absolute quantity.

Sirtuin activity. Sirtuins are a family of enzymes that consume NAD+ as a substrate. This is the thread that connects NAD+ to a large body of research on cellular ageing, and it is where most of the recent literature sits.

NAD-dependent repair pathways. A separate set of enzymes also consume NAD+ during DNA-damage response. Research in this area investigates the relationship between that consumption and the size of the available cellular pool.

Research suggests these three demands compete for the same finite pool, which is why so much of the current work is framed around availability rather than any single pathway. We are describing what the literature investigates — not asserting an outcome.

Specifications

On that 616mg figure: the certified quantity is above the 500mg label. That is overfill, it is deliberate, and it is a pass — our certificate logic only fails a lot in the underweight direction, below 90% of label. You are not getting less than you paid for.

Lab testing and this lot's certificate

This lot was tested by Vanguard Laboratory, an independent A2LA-accredited lab (A2LA certificate #6377.01.01), and the full certificate is published — no request needed, no email, no gate. Laboratory ID V260618-8 008, reported 1 July 2026.

Three assays, three results on the document:

• Chromatographic purity by HPLC-UV/VIS — ≥99.8 Area%, one clean peak.
• Quantity / assay — 616mg against a 500mg label.
• Endotoxin by LAL — <5.00 EU/mg.

Signed by Dustin Newman, Laboratory Director; approved by Tori Johnson, Operations Manager.

A note on our own record-keeping, because we would rather you heard it from us: an earlier certificate for a NAD+ 500mg lot (V260407-4 024) was internally inconsistent — it carried a 500mg title against a 1200mg quantity and a mismatched lot suffix. Vanguard identified the error and reissued. The certificate linked from this page is the corrected one, V260618-8 008, and its lot matches the vial.

Verify any batch on the lab tests page →

Handling and storage

Lyophilized. Reconstitute with bacteriostatic water; keep cold and protected from light.

NAD+ deserves a little more care than the peptides sitting next to it. It is more sensitive to light and to warmth than a lyophilized peptide, so "keep cold and dark" is not boilerplate on this page — it is the actual handling instruction. Reconstituted solution should be treated as a short-lived working preparation, not a stock you keep for weeks.

The reconstitution reference on this page will give you concentration for a given diluent volume. As with every calculator on this site: it is a reference for in-vitro laboratory handling only. Its outputs describe the physical properties of the reconstituted vial — concentration and mass drawn — and are not dosing guidance, administration instructions, or medical advice.

Diluent is sold separately: bacteriostatic water, 10ml. It is free with your first subscription order.

Questions

How much bacteriostatic water does a 500mg vial take?

That is a choice, not a fixed number — it depends on the working concentration your protocol needs. The reconstitution reference on this page converts any diluent volume into the resulting concentration. We publish the physical relationship; we do not publish a recommended volume, because a recommended volume would be a protocol.

Why is the certified quantity 616mg on a 500mg vial?

Deliberate overfill. Our COA status engine passes any lot at or above 90% of label mass with no upper bound, so 616mg is a plain pass. Fill above label protects against losses in handling.

Is NAD+ a peptide?

No. It is a dinucleotide. It is in the catalog because researchers working on cellular-energy questions ask for it, not because it belongs to the peptide class.

What is the difference between the 500mg and 1000mg vials?

Mass only — same molecule, same lot-level testing regime. The 1000mg is the better unit economics if your work consumes it at volume.

Does the certificate cover my exact vial?

It covers lot VR060152601500. Match the lot on your vial to the lot on the certificate. If they differ, look yours up on the lab tests page.

Research Use Only. This product is sold for in-vitro research use only. It is not a drug, not a supplement, and not for human or veterinary use. Hello Stacks makes no claims regarding the safety, efficacy or suitability of any compound for any use, and publishes no dosing guidance.